The American Association of Feline Practitioners (AAFP) recommends screening all cats for FeLV infection at the time they are acquired, before initial vaccination, after potential exposure to FeLV-infected cats, and as part of a diagnostic workup for any illness.
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Two types of blood tests commonly used to diagnose FeLV detect a protein component of the virus called FeLV P27. One of these tests, called an enzyme-linked immunosorbent assay (ELISA), is usually performed first as a screening tool, and can be run in a veterinarian's office.
Diagnosis is usually performed by clinicians using the enzyme-linked immunosorbent assay (ELISA) or lateral flow tests that detect the p27 FeLV antigen. The aim of this work was the development of the FeLVCHECK Ag ELISA, a new rapid direct sandwich ELISA assay that detects the p27 antigen of FeLV.
Diagnosis of FeLV infection typically begins with a positive result on a screening test, such as the SNAPTM Feline TripleTM Test and SNAPTM FIV/FeLV Combo Test, which evaluate for the p27 antigen.
